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Antibodies bound to the blocking peptide no longer bind on the epitope to the concentrate on protein. By evaluating the staining from the blocked antibody versus the antibody by itself, one can see which staining is precise.
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This guidebook outlines additional feasible optimization of cellular and immunoassay parameters to be sure the absolute best success are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind specially into the goal antibody and block antibody binding. These peptide commonly consists of the epitope acknowledged with the antibody.
Focus on Information and facts Cyclin E belongs to the extremely conserved cyclin spouse and children, whose users are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic function. Cyclin E sorts a complex with and capabilities as a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is degraded as cells progress by way of S phase.
Overexpression of this gene has been noticed in several tumors, which results in chromosome instability, and thus may possibly add to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a important role in advertising cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described. Two further splice variants ended up claimed but in depth nucleotide sequence information and facts isn't nevertheless available.
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